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The seed shattering trait has been repeatedly reshaped during rice evolution. Reduced in cultivated rice and increased in weedy rice, shattering is of great agronomic importance because of its association with yield losses. Since its first descriptions, the phenotypic patterns and the genetic regulation of cultivated and weedy rice seed shattering have been extensively studied, with a variety of methods and techniques. The aim of this review is to discuss and recommend the most suitable experimental methods for phenotypic and molecular evaluation of seed shattering in cultivated and weedy rice. Rice seed shattering must be quantified, preferably, by breaking tensile strength (BTS) assays, because other methods are more prone to human errors. The evaluation time is particularly important, and the developmental stages of the panicles measured need to be recorded. QTL analyses and GWAS studies are suitable for discovery of genes influencing shattering, but the resulting genes may only be relevant in the parental lines or the populations used. The variety of cultivated rice and evolutionary origin of weedy rice accessions has a great influence on results of rice seed shattering phenotypic and genotypic analyses and needs to always be taken into account.